(PDF) Rhamnolipid production from waste cooking oil using newly
the deg radation of soybean oil and the production of f atty acids. During the 96 h fermentation, the oil degradation rate reached more than 85.20%, the cell density at OD 600 was 2.32, and the
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Microbial production of rhamnolipids: opportunities, challenges
Production of rhamnolipids in Pseudomonas involves several steps. The precursors for rhamnolipid synthesis are the sugar (dTDP- l -rhamnose) and hydrophobic moieties such as 3- (3-hydroxyalkanoyloxy)alkanoic acid (HAA). The sugar moiety can be synthesized from d -glucose, while the hydrophobic moiety can be synthesized through the fatty acid
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Physicochemical and Antimicrobial Properties of New Rhamnolipids
Abalos et al. [56] isolated six rhamnolipids from P. aeruginosa AT10 cultures produced on soybean oil refinery byproduct and assessed the antibacterial activities of the solution and at doses
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Microbial production of rhamnolipids using sugars as carbon sources
Carbon sources including sugars, glycerol, n-alkanes, oils and polycyclic aromatic hydrocarbons have been used for microbial production of rhamnolipids with various yields [ 41 ¨C 45 ]. Currently, the highest rhamnolipids yield is obtained from oil-type carbon sources as they can be easily degraded through the ¦Â-oxidation pathway [ 46 ].
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Production of Rhamnolipids from Soybean Soapstock ...
Abstract In this study, waste from the soybean industry was used as the main carbon source to produce rhamnolipids. This biosurfactant have a great value and can be used in several applications. The biosurfactant produced in this study was characterized through to HPLC¨CUV, and the emulsification index was determined. The work also made a biodegradation test of lubricating oil residue, using
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Rhamnolipids: Pathways, Productivities, and Potential
The composition of rhamnolipid congeners, detected by high-performance liquid chromatography (HPLC)-mass spectrometry (MS) and mass percentages quantitated by HPLC-evaporative light-scattering detection (ELSD), is given in Table 5.1 for a rhamnolipid mixture produced from soybean oil by P. aeruginosa E03-40 (Dashtbozorg et al., 2016). Rhamnosyl-¦Â-hydroxydecanoyl-¦Â-hydroxydecanoate (R-C10-C10
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Microbial production of rhamnolipids using sugars as carbon sources
Rhamnolipids are a class of biosurfactants with effective surface-active properties. The high cost of microbial production of rhamnolipids largely affects their commercial applications. To reduce the production post, research has been carried out in screening more powerful strains, engineering microbes with higher biosurfactant yields and exploring cheaper substrates to reduce the production
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Production of rhamnolipids in solid-state cultivation using a mixture
4, and glycerol or soybean oil. The amount of glycerol or soybean oil is quoted as a percentage (v/v; volume of glycerol or soybean oil per total volume of impregnating solution). The inoculated
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Rhamnolipids in perspective: gene regulatory pathways, metabolic
It was shown that overexpression of estA gene affected the rhamnolipid production and recovery in the extracellular milieu. The SG81estA+ clone of P. aeruginosa showed a rhamnolipid production of 18.90 g/10 9 cells while the wild type strain produced 14.94 g/10 9 cells. Interestingly, both groups have shown the overexpression of EstA resulted
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Exploring the world of rhamnolipids: A critical review of their
In the case of rhamnolipids, the source of nitrogen for the optimization of the production is in the form of nitrates. The optimal production of rhamnolipids can be achieved with a carbon to nitrogen ratio in the range of 18:1 to 16:1, whereas the production of rhamnolipids is inhibited with a carbon to nitrogen ratio of less than 11:1 [68].
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Physicochemical and Antimicrobial Properties of New Rhamnolipids
The rhamnolipids produced by Pseudomonas aeruginosa strains are often a mixture of several homologues. Up to seven (R2C10C10 + R1C10C10 + R2C10C12 + R1C10C12 + R1C12:1C10 + R1C12:2 + R1C8:2 ) have been identified in cultures of P. aeruginosa AT10 from soybean oil refinery wastes. This study deals with the production, purification, and identification of rhamnolipids in the mixture (M7), as well
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Maximize rhamnolipid production with low foaming and high yield
Optimal production at 10% DO, pH 5.7 and with soybean oil (SBO) as substrate. 42 g/l rhamnolipids obtained with 47% yield and 220 mg/l-h productivity. Foaming halved and rhamnolipid yield tripled with SBO instead of glycerol as substrate. SBO gave primarily (82%) monorhamnolipids; glycerol more dirhamnolipids (64%).
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Rhamnolipids: Production, Performance, and Application
Rhamnolipids produced by P. aeruginosa SP4 (Pornsunthorntawee et al. 2008) were found to exhibit excellent emulsification properties for vegetable oils (palm oil, soybean oil, coconut oil, and olive oil) but failed to emulsify short-chain hydrocarbons (pentane, hexane, heptane, toluene, and 1-chlorobutane). 3.4 Foaming Behavior
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Microbial production of rhamnolipids: opportunities, challenges
Rhamnolipids are a class of biosurfactants which contain rhamnose as the sugar moiety linked to ¦Â-hydroxylated fatty acid chains. Rhamnolipids can be widely applied in many industries including petroleum, food, agriculture and bioremediation etc. Pseudomonas aeruginosa is still the most competent producer of rhamnolipids, but its pathogenicity may cause safety and health concerns during large
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Rhamnolipids produced under aerobic/anaerobic conditions: Comparative
Generally, oxygen is crucial in the growth and metabolism of bacteria, and the production of rhamnolipids is usually proportional to the oxygen level, especially in industrial mass fermentation. Most of the existing studies concentrate on rhamnolipids production via P. aeruginosa under aerobic conditions. Compared to the synthesis of
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Structural and Physicochemical Characterization of Rhamnolipids
Rhamnolipids are important biosurfactants for application in bioremediation, enhanced oil recovery, pharmaceutical, and detergent industry. In this study, rhamnolipids extracted from P. aeruginosa P6 were characterized to determine their potential fields of application. Thin-layer chromatographic analysis of the produced rhamnolipids indicated the production of two homologues: mono- and di
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Production of rhamnolipid biosurfactants in solid-state fermentation
The rhamnolipid production from soybean meal as the renewable carbon source by Pseudomonas aeruginosa (PTCC 1074) under solid state fermentation was studied for the first time. A two-step experimental design procedure using FFD and RSM was used to screen and optimize different nutritional and environmental parameters to improve the efficiency.
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Foaming of rhamnolipids fermentation: impact factors and fermentation
Many aspects of the medium compositions affect foam formation, including soybean oil, fresh medium addition (except soybean oil), etc. Soybean oil as a carbon source consisted of longer chain fatty acids is widely applied in the rhamnolipids fermentation [11, 59,60,61], besides it has excellent defoaming properties and can compete with foaming
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Physicochemical and Antimicrobial Properties of New Rhamnolipids
This study deals with the production, purification, and identification of rhamnolipids in the mixture (M7), as well as their physical and chemical characterization and the evaluation of their antimicrobial properties. The rhamnolipids produced by Pseudomonas aeruginosa strains are often a mixture of several homologues. Up to seven (R2C10C10 + R1C10C10 + R2C10C12 + R1C10C12 + R1C12:1C10 + R1C12
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