Lipase from marine strain using cooked sunflower oil waste
The marine strain Pseudomonas otitidis was isolated to hydrolyze the cooked sunflower oil (CSO) followed by the production of lipase. The optimum culture conditions for the maximum lipase production were determined using Plackett-Burman design and response surface methodology.
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Lipase production by Yarrowia lipolytica using olive oil
Though two studies reported the production of lipase using by-products from olive oil extraction (Moftah et al., 2013; Lopes et al., 2016), to our knowledge, rapeseed cake (RC) and sunflower cake
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Biodiesel production from waste cooking sunflower oil
The selected strain was monitored during a batch cultivation for 192 h on 100 g/L carbon source: pure glycerol as a hydrophilic source, sunflower waste cooking oil as a hydrophobic source, and
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Sunflower Seed Lipase: Extraction, Purification,
Thus, the prepared enzymes L1 and L2 can be used for effective production of oleic acid from olive oil. Similarly, lipase isolated from sunflower seeds showed a high hydrolysis rate on olive oil
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Production of new lipase from Preussia africana and partial
Results show that an optimal lipase production of 14.5 kDa has been attained after 48 h of fermentation when sunflower oil was used in the C/N ratio of 8.63 in the fermentation medium. The produced lipase showed greater activity for oils that had higher percentage of unsaturated fatty acids in their composition.
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Review: Lipase-catalyzed process for biodiesel production
Lipase LA201 Thermomyces lanuginosa Sunflower oil 2-Propanol 3 40 72.8 Deng et al. (2005) Lipase PS Pseudomonas cepacia Mahua oil Ethanol 4 40 96 Kumari et al. (2007) Soybean oil Methanol 7.5 35 67 Noureddini et al. (2005) Sunflower oil 1-Butanol 3 40 88.4 Deng et al. (2005) Lipozyme RM IM Rhizomucor miehei Sunflower oil Methanol 3 40 >80
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A Review on Microbial Lipases Production - Food
This review paper provides an overview regarding the main aspects of microbial lipases production. The most important microbial lipase-producing strains for submerged and solid-state fermentations are reviewed as well as the main substrates, including the use of agroindustrial residues. Current process techniques (batch, repeated-batch, fed-batch, and continuous mode) are discussed and the
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Biodiesel Production from Crude Sunflower Oil and Crude
The aim of this research was to study the immobilization of lipase on chitosan by adsorption in order to examine the use of lipase as a catalyst in the transesterification of crude sunflower oil and Jatropha oil. The highest activity of immobilized lipase was found to be 857 U/g, and it corresponded to a glutaraldehyde concentration of 0.05%, pH 7, and a temperature of 37 ¡ãC. On the other
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Biodiesel production from sunflower, soybean, and waste
Thermomyces lanuginosus lipase was covalently attached onto MPPM with 80%, 85%, and 89% immobilization efficiencies using bead, powder, and monolithic forms, respectively. Immobilized enzymes were successfully used for the production of biodiesel using sunflower, soybean, and waste cooking oils.
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Olive and sunflower cakes as suitable substrates for lipase
Oil cakes, such as olive and sunflower cakes, are generated during vegetable oil extraction and have received great interest as solid substrates in SSF processes. In the present work, a 50% (w/w) mixture of olive and sunflower cakes was used as substrate in SSF for lipase production by several strains of Y. lipolytica and Y. divulgata.
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Optimization of critical medium components for lipase
During recent research, sunflower oil was used as an inducer. It was not remarkable factor for the production of lipases. The lipase production of Neurospora crassa decreased with an increase in the concentration of sunflower oil. Maximum lipase activity was observed at 2.5 ml sunflower oil which was 2.5 ¡À 2.20 a U/ml.
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Effect of Plant Oils upon Lipase and Citric Acid Production
Among tested plant oils, olive oil proved to be the best medium for lipase and CA production. The Y. lipolytica DSM 3286 produced 34.6 ¡À 0.1 U/mL of lipase and also CA and SCP as by-product on olive oil medium supplemented with yeast extract. Urea, as organic nitrogen, was the best nitrogen source for CA production.
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Silk©Cocoon Matrix Immobilized Lipase Catalyzed
The reason for the lower Km value of the immobilized lipase than the free lipase is attributed to the hydrophobic [24, 25] and porous nature of the silk-cocoon matrix that provides nonaqueous environment for fast diffusion of the less polar substrates, sunflower oil and methanol, which resulted in effective enzyme-substrate interaction for the
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Utilization of oleochemical industry residues as substrates
The obtained results showed that the lipase activity achieved was 10 U/ml. The produced lipase has been used in sunflower oil hydrolysis. The results revealed that 39.5% of the original tested sunflower oil was hydrolyzed into free fatty acids after 24 h at 37 ¡ãC.
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Microbial lipases: An overview of screening, production
In commonly, oil carbon source such as vegetable oil, olive oil, coconut oil is common influencing factors in SmF system due to their sticky nature, even though various oils as a carbon source have been utilised to produce lipase enzyme from microbial strains especially olive oil, palm oil, sunflower oil, and almond oil has enhanced higher
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Immobilized lipase©catalyzed ethanolysis of sunflower oil
The Journal of the American Oil Chemists' Society publishes original research and technological advances on fats, oils, oilseed proteins, and related materials. Abstract The ethanolysis of sunflower oil (SFO) with Lipozyme (immobiliz¨¦d 1,3-specific Mucor miehei lipase) in a medium solely composed of substrates was investigated.
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Production of lipase from Micrococcus flavus and influence
sunflower oil (Fig - 1) and palm oil (Fig - 2) . The higher production of lipase enzyme was 18.4935 IU/L in sunflower oil and 12.1832 IU/L was in palm oil as substrate. From the values of lipase assay, the maximum lipase production recorded at pH 8 when sunflower oil used as substrate and in palm oil the higher production at pH 7.
Get InquiryExtracellular Lipase Production by Fungi from Sunflower Seed
Sunflower oil in the. inhibited, stimulated or had no effect on lipase production, depending on the isolate. of both field and storage fungi produced extracellular lipases which were active against. but generally more activity was observed in storage fungus filtrates than in field fungus. Key Words: extracellular fungal lipase, sunflower seed
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Olive and sunflower cakes as suitable substrates for lipase
Oil cakes, such as olive and sunflower cakes, are generated 22 during vegetable oil extraction and have received great interest as solid substrates in SSF 23 processes. In the present work, a 50 % (w/w) mixture of olive and sunflower cakes was 24 used as substrate in SSF for lipase production by several strains of Y. lipolytica and Y.
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